ANTI-CANCER POTENTIAL OF COPPER OXIDE NANOPARTICLES AGAINST MURINE MAMMARY ADENOCARCINOMA (AMN-3) CELLS

This study was designed to evaluate the cytotoxic effect of copper nanoparticles in murine adenocarcinoma cells (AMN-3). The exposure period cell line performed at 24 hr a microtitration plate under complete sterile conditions. Different concentrations were used started from 0.39 μgmL-1to 50 μgmL-1 three independent experiments. First, stained by MTT and absorbance measured using Elisa reader 492 nm. treatment with showed significant inhibition (P< 0.05) on concentration dependent, highest 88% μgmL-1, while 15% inhibitory 1.5 μgmL-1. death evaluated after CUNPs through two types assessments, which mitochondrial membrane potential assay acridine orange- ethidium bromide dual staining assays. Results revealed that tested substances potent against proliferation AMN-3 apoptosis.